No. This would be considered a prohibited transaction so is not allowed.
This is a question to consult with your Tax Advisor. Technically you can fund it with a personal account BUT, the purpose of your 401k as the business owner is to mainly save money from the profits from your business is making and have a business tax write off. Also to make your business more competitive in the hiring market to bring talent to your business.
Hope this helps, David Kleinik